Purification, Structural Elucidation, and Anticancerous Properties of a Novel Flavonoid from Flowers of Leucas indica

Previously, we showed that the crude methanol extracts of Leucas indica flowers exhibited antioxidant properties and in current study, flower L. anticancerous as evidenced cytotoxicity (MTT assay test) against selected cancerous cell lines HeLa, HCT116, HL-60, MCF-7. Therefore, further analysis was performed to isolate purify bioactive compound using activity-guided repeated fractionation extract by silica gel column chromatography. After collection different fractions, all fractions were subjected TLC which yielded same compounds on analyzed for physicochemical spectroscopic analyses, e.g., UV, IR, 1H NMR, 13C COSY, HSQC, mass spectroscopy. The isolated elucidated 6-hydroxy-3-(4-hydroxyphenyl)-7-(3,4,5-trihydroxy-6-)(hydroxymethyl)tetrahydro-2H-pyran-2yl)-4H-chromen-4-one. Based properties, might be a promising source useful natural products newly offer opportunities develop new drugs.